Results for "Genetics & Molecular Biology"

New Plasmid Platform 'pGGTOX' Revolutionizes Genome Editing in Diverse Enterobacteriaceae

Scientists have developed pGGTOX, a versatile and highly efficient plasmid platform that enables precise, marker-free genome editing across a wide range of Enterobacteriaceae. This system integrates multiple advanced features to streamline bacterial genetic modifications, including targeted gene deletions and the insertion of complex modules like CRISPR–Cas9 for curing antibiotic resistance. pGGTOX significantly expands the toolkit for studying bacterial pathogens and combating antimicrobial resistance.

By Liu, Tian, Cui, Liu, Feng, Liu, Wang, Shi, Chen, Hao